FastQCFastQC Report
Mon 5 Aug 2019
8C_32psu_2_S6_L001_R1_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_32psu_2_S6_L001_R1_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12936990
Sequences flagged as poor quality0
Sequence length20-150
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATGCC2000.075.24164140-144
CAAGCTG607.743438E-963.7963453
CGGTGCT501.298858E-463.361378140-144
TGCCCTG352.2945278E-762.307106140-144
GGCCTGC600.060.57635140-144
TGCCAGT251.9851017E-458.1533140-144
AGACACT32300.057.7299041
GACACTG32350.057.640682
CGGGGCA701.0054451E-556.57266140-144
CGCCATG850.00567666455.262707140-144
GACTTGC455.314578E-755.1762140-144
TGCTGCA200.005989683354.51872140-144
GAGCACG1203.3863963E-452.192562140-144
GTCCCTG351.6708496E-551.92259140-144
GGCCGCT504.6124114E-850.884136140-144
GTGCCCG504.6124114E-850.884136140-144
ACACTGC36850.050.1566123
CACTGCC37000.049.8054854
TCTTCCG146900.049.6945152
AAGCCTG250.00866444649.65858140-144
Produced by FastQC (version 0.11.5)