FastQCFastQC Report
Mon 5 Aug 2019
8C_32psu_1_S5_L002_R2_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_32psu_1_S5_L002_R2_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13959529
Sequences flagged as poor quality0
Sequence length15-145
%GC29

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG198020.14185292354777873No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCCTG150.001584844239.26384138-139
CGGACCT200.003748829179.44788138-139
GCCGGGC250.007306657143.5583138-139
CGCAGCA550.00355621162.19195132-133
CCGGAGC1601.7462298E-1048.761913118-119
GTACTGC500.00965843448.31195480-81
AGACACT19200.048.1346361
GGTCCCC1056.763628E-445.361534134-135
GTGGTCC1300.002392386844.431282138-139
GACACTG21550.042.885162
TCTTCCG160600.040.391742
CACTGCC24200.037.823614
ACACTGC24450.037.6177183
TGCCACA24700.036.8789257
TTCCGAT159950.036.8513954
CTGCCAC24850.036.8342556
ACTGCCA24900.036.7602965
CTTCCGA171250.036.1755833
CGATCTG14700.033.6905947
GGTATGC15550.033.5551832
Produced by FastQC (version 0.11.5)