FastQCFastQC Report
Mon 5 Aug 2019
8C_32psu_1_S5_L002_R1_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_32psu_1_S5_L002_R1_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13972671
Sequences flagged as poor quality0
Sequence length15-145
%GC29

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCCG100.003366659186.01567120-121
CAGGCAA150.007868072140.04785126-127
AGACCGG401.4942183E-4138.09375138-139
CGGCATC400.001392685978.776924126-127
AGACACT31500.063.3447651
AAGCCAG550.003573139262.11744130-131
CTGCTGC1201.5203703E-461.374996138-139
TGCAGAC650.00385858660.92366136-137
GCAATTG750.006798179452.8005136-137
GGGCTGG2107.3840783E-752.60714138-139
CACGGAG1050.006906361352.589794120-121
AGACTGG650.00692161152.560913130-131
CAAGCTG1200.051.2754063
GCCAGAC700.00789965650.832817132-133
ATTGCCC1100.00829000950.215904138-139
ACAGAGG1800.00857841649.78257138-139
GACACTG41700.047.7449652
CCAATGC1005.4213963E-447.44397132-133
GCCGGAT1451.5774585E-647.123573130-131
ACACTGC46000.043.3770833
Produced by FastQC (version 0.11.5)