FastQCFastQC Report
Mon 5 Aug 2019
8C_32psu_1_S5_L001_R1_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_32psu_1_S5_L001_R1_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13992527
Sequences flagged as poor quality0
Sequence length15-145
%GC29

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCAGC150.0014544703246.22362138-139
GCATGCA300.004138275173.61487136-137
ACTGGCA100.0048464877164.68579114-115
GCGCATG400.006397565150.08055138-139
AGCAGCT250.006706383147.73416138-139
TGCTTGC200.009160647133.09917136-137
GCTAGAC450.001108416683.43919134-135
CTGCTAG1256.002665E-1176.69858132-133
GGCTGCG450.00300456164.89494122-123
AGACACT31700.062.511421
CGTCAGC650.004763901757.7656134-135
CACAGGT702.4727586E-455.624237122-123
TGCCTTC1003.070081E-453.239666136-137
GCGGCTG650.00785004550.913948128-129
ACAGGTC600.0079661650.725227124-125
GACACTG42000.046.9720082
CAAGCTG754.7639332E-946.8673933
CGATCTG12200.045.738937
GATCTGC3150.043.240758
TCTTCCG159150.042.764842
Produced by FastQC (version 0.11.5)