FastQCFastQC Report
Mon 5 Aug 2019
8C_26psu_4_S12_L002_R2_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_26psu_4_S12_L002_R2_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12457033
Sequences flagged as poor quality0
Sequence length15-145
%GC26

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG988050.7931663984513807No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA128910.10348371076804563No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACACTG14350.053.6493532
ATGCGCA200.00727026551.91222478-79
AGACACT15300.050.691321
CAAGCTG350.00922228548.8805243
TCTTCCG91000.045.621822
CACTGCC17000.045.621824
TGCCACA17000.045.2863667
CTGCCAC17100.045.0215346
ACACTGC17400.044.5730443
ACTGCCA17650.043.6185955
GATCTGC1700.043.6090938
TTCCGAT95050.041.6980064
CTTCCGA102650.039.444093
GCCACAT19250.039.1044168
GCCGATC2250.038.018186
CCGATCT99600.037.4456186
TCCGATC108100.034.553995
GGTATGC7250.033.8230742
TCCGATG1358.5197826E-833.793945
CGATCTG5750.033.7204747
Produced by FastQC (version 0.11.5)