FastQCFastQC Report
Mon 5 Aug 2019
8C_26psu_4_S12_L002_R1_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_26psu_4_S12_L002_R1_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12474515
Sequences flagged as poor quality0
Sequence length15-145
%GC26

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACACT27800.079.373571
AGGCACT305.3370793E-575.8276751
GACACTG33700.064.971042
ACACTGC38000.057.767563
CACTGCC38650.057.090334
TGGATCC402.2165933E-456.8696178
TCTTCCG123800.056.1816982
CTGCCAC41250.053.078316
TGCCACA41400.053.023367
ACTGCCA41800.052.6520165
TTCCGAT125400.051.6996544
GGCACTG453.9648748E-450.5517852
CAAGCTG453.9652668E-450.5507743
GCACTGC453.9652668E-450.5507743
CTTCCGA134350.049.8640943
GCCACAT44500.049.3295978
CGGATCC350.00932297548.7463651
CTTCCGG2000.048.3391763
CGATCTG6900.047.8034447
CCGATCT132850.045.5042536
Produced by FastQC (version 0.11.5)