FastQCFastQC Report
Mon 5 Aug 2019
8C_26psu_4_S12_L001_R2_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_26psu_4_S12_L001_R2_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12561390
Sequences flagged as poor quality0
Sequence length15-145
%GC26

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1036950.825505776032748No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA145270.11564802939802044No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCTTG250.00243021368.463095
GCCCAGC300.00500421857.052354
AGACACT15000.052.109681
GACACTG15250.052.0024382
CTGCCAC17500.045.9680756
CACTGCC17550.045.186764
TCTTCCG95300.045.139542
ACACTGC17800.044.8726353
TGCCACA17900.044.622137
ACTGCCA18800.042.4859625
TTCCGAT97400.041.76424
CTTCCGA104800.040.012863
GCCACAT20000.039.9368068
CGATCTG7250.036.985817
CCGATCT105250.036.0474746
TCCGATC110650.034.6491975
GGTATGC8000.032.092332
CAGCGGC750.006927813428.232393130-131
GACATTC14800.028.1407998
GCCGATC3050.028.0586456
Produced by FastQC (version 0.11.5)