FastQCFastQC Report
Mon 5 Aug 2019
8C_26psu_4_S12_L001_R1_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_26psu_4_S12_L001_R1_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12580524
Sequences flagged as poor quality0
Sequence length15-145
%GC25

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACACT29850.077.074121
AGACCGG552.1600335E-673.31166138-139
CAAGCTG501.2108649E-768.336513
GACACTG36500.062.407772
TCTTCCG120750.058.0082132
ACACTGC39950.056.8758163
CACTGCC40850.055.9015464
CGATCTG7300.054.6067967
TTCCGAT123650.054.11474
CTGCCAC43550.052.3050166
ACTGCCA43550.052.1742555
TGCCACA43700.051.9951677
CTTCCGA133100.051.3850143
GACCCGG300.00806121750.57358124-125
GCCACAT45150.049.568568
CCGATCT129300.047.5660136
TCCGATC134250.046.0242775
AAGCTGG803.1042182E-642.7103164
CCGATTG801.5507618E-435.591936
CTCTTCC216700.033.6899721
Produced by FastQC (version 0.11.5)