FastQCFastQC Report
Mon 5 Aug 2019
8C_26psu_3_S11_L002_R2_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_26psu_3_S11_L002_R2_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17150712
Sequences flagged as poor quality0
Sequence length15-145
%GC29

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG220080.12832120322468246No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCTGC100.0032773314187.69543132-133
TGCCCCG150.008079791138.80965136-137
AAGCCGG700.002691589866.721016122-123
AGACACT28500.054.0572851
CGGCTCA550.007684373351.189663132-133
GGAGCTC1400.00797241950.714687138-139
GACACTG31100.048.923842
ACACTGC34500.043.6874773
GATCTGC2950.043.6550528
CACTGCC34550.043.4862024
CTGCCAC34850.042.427796
TGCCACA34850.042.2909247
ACTGCCA35700.042.085395
CGATCTG13150.041.712257
TCTTCCG190250.041.4668542
TTCCGAT185650.039.2570274
GCCACAT39700.037.605098
CTTCCGA202050.037.5342063
CACGTGG1050.00260510834.478817130-131
CCGATCT187750.034.1183176
Produced by FastQC (version 0.11.5)