FastQCFastQC Report
Mon 5 Aug 2019
8C_26psu_3_S11_L002_R1_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_26psu_3_S11_L002_R1_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17169174
Sequences flagged as poor quality0
Sequence length15-145
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACACT48900.068.429461
CAGGCGC400.002996591664.938705126-127
CACGTGG700.003218896263.77615138-139
CAGAGGC450.0035800662.08743130-131
GGCGCTG500.004031817460.25223134-135
CTAGTGC550.0046669858.06663136-137
TGCCACG250.00503768456.9564447
GGCCGTC400.006230883853.97718116-117
GGAACTG1200.007156827552.11831136-137
ATCTGGC558.240513E-751.7788939
GACACTG64300.051.5975572
GCTTGAC703.676688E-451.33062128-129
TGTTCCG750.0090084249.1714108-109
TCTTCCG204100.046.7894442
ACACTGC71000.046.728353
CACTGCC71400.046.533044
ACTGCCA74150.044.6152425
CTGCCAC74450.044.371716
TGCCACA74200.044.1374057
TTCCGAT196950.042.9691734
Produced by FastQC (version 0.11.5)