FastQCFastQC Report
Mon 5 Aug 2019
8C_26psu_3_S11_L001_R2_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_26psu_3_S11_L001_R2_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17194922
Sequences flagged as poor quality0
Sequence length15-145
%GC29

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG238340.1386106898304046No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGCGC255.8899214E-497.831955124-125
AGACACT29650.054.7243041
GACACTG34150.047.233292
GGAGCAC605.64107E-447.063717116-117
TCTTCCG192900.042.6260722
ACACTGC37950.042.252243
CACTGCC37700.042.026094
CGATCTG13150.041.7345357
TTCCGAT190500.040.232114
ACTGCCA39200.040.1745835
TGCCACA39300.040.0723577
CTGCCAC39800.039.4490286
CTTCCGA206850.038.390173
TCCGGTC500.001579772638.1779174
GCCACAT44300.035.765078
CCGATCT196550.034.4048966
GATCTGC4200.032.951378
CCGTAGC1800.003265811832.92465138-139
GCGAGCT650.0034265632.603645100-101
ATCTGGC752.996877E-431.8151159
Produced by FastQC (version 0.11.5)