FastQCFastQC Report
Mon 5 Aug 2019
8C_26psu_3_S11_L001_R1_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_26psu_3_S11_L001_R1_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17214759
Sequences flagged as poor quality0
Sequence length15-145
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGCGC100.0064755655149.47646118-119
CAGTCAG400.001899298272.852936132-133
AGACACT49100.067.435281
GTGGACC250.003580678562.08495398-99
GCTTGAC550.004582671458.33464136-137
ATCTGGC504.2848478E-756.9238788
AAAGGCC400.00536645856.05367118-119
CCGGCCA250.006298307353.83064390-91
GACACTG64850.050.7648052
CTGGCCA205.423436E-447.4411110-11
ACACTGC69450.047.4024123
CACTGCC69750.047.1305244
TCTTCCG199800.046.89052
TGCCACA73050.045.1312987
CTGCCAC73200.045.103626
ACTGCCA73200.044.974015
CGATCTG11700.044.193047
TTCCGAT188850.043.832044
GCCACAT78150.042.1253668
CTTCCGA210300.041.729743
Produced by FastQC (version 0.11.5)