FastQCFastQC Report
Mon 5 Aug 2019
8C_26psu_2_S10_L002_R2_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_26psu_2_S10_L002_R2_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18360883
Sequences flagged as poor quality0
Sequence length15-145
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG292330.15921347573534453No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGGGC150.001937022572.4930780-81
GCATGTC400.00723250251.98055130-131
AGACACT27700.048.9183351
GACACTG30700.044.6434442
CACTGCC33950.039.30344
ACACTGC34400.039.240293
CGATCTG10450.037.6138237
ACTGCCA35900.037.1685335
TGCCACA36000.036.7779587
CTGCCAC36250.036.6669926
TCTTCCG179750.036.3112032
CCGATGC600.00260498534.479345
TTCCGAT177150.034.2457774
GCCACAT39350.033.5154958
CTTCCGA191000.032.8997843
GATCTGC3250.031.8270828
GGTATGC17800.031.0895142
CCGATCT183050.030.3165786
GCCGATC6700.030.1050936
TCCGATC199350.028.2009335
Produced by FastQC (version 0.11.5)