FastQCFastQC Report
Mon 5 Aug 2019
8C_26psu_2_S10_L002_R1_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_26psu_2_S10_L002_R1_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18381264
Sequences flagged as poor quality0
Sequence length15-145
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTGCC150.00210980970.9481878-79
ACTGAGC300.002163163470.50264130-131
AGACACT56400.057.639841
GACACTG59400.054.6420022
ACACTGC67400.048.3086553
CACTGCC67700.047.7140924
TCTTCCG199500.046.0448382
CTGCCAC71150.045.5452966
TGCCACA71350.045.489837
ACTGCCA71750.045.020825
CGATCTG11050.043.3599177
TTCCGAT195100.042.7520874
GCCACAT76850.042.3682948
CTTCCGA207950.041.8445133
CAAGCTG1308.54925E-1139.630033
CCGATCT197600.038.2221266
TCCGATC206850.037.0608335
GCCGATC5850.036.1073656
ATCTGGC600.002654939734.3460279
CTTCCGG8350.031.4667223
Produced by FastQC (version 0.11.5)