FastQCFastQC Report
Mon 5 Aug 2019
8C_26psu_2_S10_L001_R2_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_26psu_2_S10_L001_R2_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18452692
Sequences flagged as poor quality0
Sequence length15-145
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG315550.1710048593451839No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA187080.10138358132244336No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGAGC751.9877717E-458.137127138-139
TGCCGCA300.007349762651.7696087
AGACACT26400.049.025181
GACACTG29700.043.7514232
CGCAGTC600.00120380240.34845120-121
CACTGCC33450.038.8465544
ACACTGC33850.038.5404473
TCTTCCG184750.037.632792
TGCCACA35300.036.3707167
CTGCCAC35300.036.3707166
ACTGCCA36200.035.7524955
TTCCGAT183600.035.7255444
CTTCCGA194700.034.672613
GGTATGC17050.033.0961152
GCCACAT39250.032.9742748
CCGATCT188850.032.2651986
CGATCTG9650.030.5789387
TCCGATC202750.030.3340555
GCCGATC5550.029.8491466
CCGATGC750.00772359527.6104586
Produced by FastQC (version 0.11.5)