FastQCFastQC Report
Mon 5 Aug 2019
8C_26psu_2_S10_L001_R1_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_26psu_2_S10_L001_R1_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18475429
Sequences flagged as poor quality0
Sequence length15-145
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCCCT100.009970248129.38138124-125
AGACCGG400.001233679881.219986138-139
AGACACT57850.058.9524771
GGCCCTC350.00502211757.000946126-127
GACACTG62050.054.9621352
ACACTGC68350.049.594193
GACGGCT905.0509325E-448.13036138-139
CACTGCC69650.048.0759124
CCGGTCT651.4665584E-647.6257486
TGCCACA72650.045.9486357
TCTTCCG205600.045.672232
ACTGCCA73300.045.6115655
CTGCCAC73350.045.5101366
TTCCGAT199900.042.81914
GCCACAT77950.042.625918
CTTCCGA215000.041.3956383
GCCGATC5900.041.1007546
CTGCATG1357.67523E-540.10863138-139
CCGATCT198650.039.0627866
CGATCTG10200.038.9488377
Produced by FastQC (version 0.11.5)