FastQCFastQC Report
Mon 5 Aug 2019
8C_26psu_1_S9_L002_R2_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_26psu_1_S9_L002_R2_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18034417
Sequences flagged as poor quality0
Sequence length15-145
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACACT51500.064.323771
GCACAGG150.004581019758.34038554-55
GACACTG61600.053.252082
TCTTCCG347250.050.4438552
ACACTGC67000.048.7993283
CACTGCC66900.048.5502174
TTCCGAT344650.047.8236964
CTTCCGA359800.047.0375823
TGCCACA69250.046.5137527
ACTGCCA69900.046.3894585
CTGCCAC69900.046.1582836
CAAGCTG1104.7293724E-1044.0706863
GCCACAT73950.043.9216968
CCGATCT346350.043.840946
CGATCTG25500.043.7250337
TCCGATC363750.042.2176635
CTCTTCC454950.039.402451
GATCTGC6950.037.9761778
GATCTAC67700.036.519438
CGATCTA215750.036.1008537
Produced by FastQC (version 0.11.5)