FastQCFastQC Report
Mon 5 Aug 2019
8C_26psu_1_S9_L002_R1_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_26psu_1_S9_L002_R1_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18051351
Sequences flagged as poor quality0
Sequence length15-145
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTGCC150.002445513206.98741138-139
AGACACT87200.076.4862441
CAAGCTG1500.071.600253
GACACTG114500.057.8276862
CCTAGGC300.005900311354.726265118-119
TGCTGCT901.3274019E-553.980507138-139
GGCCACA300.006359044553.7001887
GCACTGC501.3690014E-553.7001843
ACACTGC123300.053.6566353
TCTTCCG352550.053.647172
CACTGCC125750.052.7393464
GATCTGC5900.050.969678
CTGCCAC131050.050.5244836
TGCCACA130550.050.430057
ACTGCCA131300.050.4282765
TTCCGAT341650.050.15584
CTTCCGA362850.049.4897163
GGCACTG552.4059455E-548.8186262
AGGCACT552.4059455E-548.8186261
CGATCTG21900.048.7960627
Produced by FastQC (version 0.11.5)