FastQCFastQC Report
Mon 5 Aug 2019
8C_26psu_1_S9_L001_R1_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8C_26psu_1_S9_L001_R1_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18166994
Sequences flagged as poor quality0
Sequence length15-145
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACACT88300.075.375681
AGACCGG700.002824629765.91402138-139
CAAGCTG1250.064.565953
GACACTG117300.056.694732
TCTTCCG355350.054.857282
GCTGCCA300.006310209653.8049555
ACTGCTG350.00686693352.66691128-129
AGCTCGC550.006979077652.451466138-139
ACACTGC127450.052.1373983
CGATCTG22250.051.7494857
CACTGCC129750.051.1302574
TTCCGAT350050.051.0920334
CTTCCGA368150.050.4069753
TGCCACA134250.048.9354557
ACTGCCA135400.048.7979935
CTGCCAC135200.048.7109956
CCGATCT339700.047.2793056
GCCACAT139200.047.0793348
ATCTGGC808.504685E-847.079339
TCCGATC357850.045.437635
Produced by FastQC (version 0.11.5)