FastQCFastQC Report
Mon 5 Aug 2019
16C_32psu_4_S4_L002_R2_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_32psu_4_S4_L002_R2_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15578847
Sequences flagged as poor quality0
Sequence length15-145
%GC26

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGCTG250.002407519668.625353
AGACACT26100.057.4072761
TCTTCCG244950.048.1409452
GACACTG31100.047.9936142
CGATCTG17550.045.6198967
TTCCGAT250600.044.7278864
CTTCCGA258950.044.3235783
CACTGCC34750.043.4462664
ACACTGC35800.042.0122643
TGCCACA36300.041.9062087
CTGCCAC36400.041.7910846
ACTGCCA36550.041.463115
CCGATCT256250.040.528016
GCCGATC4950.039.2805066
GATCTGC5400.039.184238
TCCGATC267400.039.1374975
CCGATGC906.773249E-638.12526
GCCACAT40850.037.2385678
CTCTTCC341750.034.973891
ATCTGCC3450.034.809969
Produced by FastQC (version 0.11.5)