FastQCFastQC Report
Mon 5 Aug 2019
16C_32psu_4_S4_L002_R1_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_32psu_4_S4_L002_R1_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15589995
Sequences flagged as poor quality0
Sequence length15-145
%GC26

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACACT46300.068.0363851
TCTTCCG231400.054.255342
GACACTG58700.053.5669172
ACACTGC60350.052.10173
CACTGCC61800.051.063934
AAGCCGG300.00788451950.857998122-123
CGATCTG13400.050.6780327
CTTCCGA237450.050.6612663
TTCCGAT228650.050.639384
CTGCCAC64300.049.1673056
TGCCACA64150.049.1043557
ACTGCCA64350.048.8630565
CCGATCT223650.046.1325156
GCCACAT68800.045.951568
TCCGATC237650.044.255285
GATCTGC4650.044.1802868
CAAGCTG550.001047876441.5025633
GATCTGG5050.039.5508358
CTCTTCC324150.039.294461
CTTCCGG6200.035.8963663
Produced by FastQC (version 0.11.5)