FastQCFastQC Report
Mon 5 Aug 2019
16C_32psu_4_S4_L001_R2_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_32psu_4_S4_L001_R2_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15691820
Sequences flagged as poor quality0
Sequence length15-145
%GC26

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGGTC400.00331361363.311485138-139
AGACACT28850.062.9188041
GCATGAA650.003662649561.730827138-139
GACACTG32950.055.436972
CAAGCTG453.8331765E-450.8991283
CACTGCC36450.050.1136444
ACACTGC36800.049.637023
TCTTCCG251950.049.2730142
CGATCTG17200.047.940187
CTGCCAC38450.047.2094046
TGCCACA38600.047.1742977
ACTGCCA38650.047.113275
TTCCGAT255000.046.527794
CTTCCGA267500.045.252653
GCCACAT41800.043.836858
CCGATCT257500.042.607296
TCCGATC267850.041.5808685
GATCTGC5000.041.2285548
GCCGATC5100.039.297376
CTCTTCC354900.035.351141
Produced by FastQC (version 0.11.5)