FastQCFastQC Report
Mon 5 Aug 2019
16C_32psu_4_S4_L001_R1_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_32psu_4_S4_L001_R1_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15704119
Sequences flagged as poor quality0
Sequence length15-145
%GC26

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGG351.7413422E-595.03464138-139
AGACCGG550.001952067672.351234138-139
AGACACT46500.068.911471
TCCGGTC605.486072E-966.638995
TGAGCTC550.00343826362.723976134-135
CAAGCTG651.0328222E-861.5129133
CGATCTG13000.060.1947827
GACACTG56450.056.7649882
TCTTCCG235150.055.4795232
ACACTGC58800.054.3991783
CACTGCC59800.053.489494
CCGGTCT657.272338E-752.7253536
TTCCGAT230000.052.6986124
CTTCCGA243150.051.6808933
TGCCACA62250.051.109017
CTGCCAC62650.050.965046
ACTGCCA63050.050.5511135
GGTCTCA350.009163870548.959268
GATCTGC3400.048.7192658
CCGATCT227050.048.2764246
Produced by FastQC (version 0.11.5)