FastQCFastQC Report
Mon 5 Aug 2019
16C_32psu_3_S3_L002_R2_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_32psu_3_S3_L002_R2_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13649015
Sequences flagged as poor quality0
Sequence length15-145
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG220430.16149883343230262No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCTGG400.003643497861.81269128-129
TGCTCGG550.0085311549.85349134-135
TAGCCGC350.00896520949.231503106-107
GACACTG11850.043.1554032
AGACACT12550.040.3640561
ACACTGC12950.039.8620873
TGCCACA13100.039.4057967
CTGCCAC13250.039.3238036
CACTGCC13150.039.255824
ACTGCCA13350.038.6677175
TCTTCCG90800.034.589382
CCAGAGC1150.00285472133.841175136-137
GCCACGA5500.033.3324284
GGTATGC10500.033.081932
TTCCGAT89650.032.6651344
CCATTGA1650.00403620831.530905114-115
CTTCCGA96900.031.4160563
GCCACAT16600.031.3879768
CGATCTG6450.030.6670347
GGGATCA1304.483706E-429.701424124-125
Produced by FastQC (version 0.11.5)