FastQCFastQC Report
Mon 5 Aug 2019
16C_32psu_3_S3_L002_R1_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_32psu_3_S3_L002_R1_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13666135
Sequences flagged as poor quality0
Sequence length15-145
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGCT100.0035953112181.97488132-133
GCTGCTG851.1224874E-681.84426138-139
GAGGCAC250.003376454363.01205100-101
CAGCGTG1002.466148E-455.654095138-139
ACCTGGT200.00810275450.5079472-73
CTGGCGG606.209871E-446.378414138-139
TCAGCTT1852.1259748E-645.124947138-139
CCGGCTG657.770286E-444.10196118-119
GACACTG24500.042.703362
AGTCCGC402.085053E-742.00333812-13
TCTTCCG95450.040.7771222
TGCCGGA800.001294922939.75389124-125
CAAGCTG853.3619654E-739.5223543
TGCTGGT2000.001420176539.013485136-137
GCCGGAT850.001487421538.64859126-127
CACTGCC27400.038.3581164
TTCCGAT92500.038.0818524
ACACTGC27550.037.8008733
AGACACT27950.037.6039921
CTTCCGA100400.036.710583
Produced by FastQC (version 0.11.5)