FastQCFastQC Report
Mon 5 Aug 2019
16C_32psu_3_S3_L001_R2_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_32psu_3_S3_L001_R2_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13665206
Sequences flagged as poor quality0
Sequence length15-145
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG239110.1749772378111241No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCGG200.00128509680.38917102-103
GGCCCTA300.0028820765.58036114-115
AGCCGAG350.00530697756.211735114-115
AGACACT11250.040.354891
GACACTG11600.038.7206462
GGGGCTC1101.129073E-437.564377128-129
GCCACGA5350.034.3042144
TGGTCAC850.002740219534.12508120-121
CACTGCC13000.033.8077054
TCTTCCG96900.033.5933842
GATCTGC1900.033.0452468
TTCCGAT93750.033.0220764
GGTATGC12050.032.865822
CCAGCAC1050.003442070432.57339130-131
CGATCTG7000.032.4279177
ACACTGC13800.032.1978153
CTTCCGA103650.030.753343
ACTGCCA14550.030.2061965
TGCCACA14600.030.1028617
CTGCCAC14600.030.1028616
Produced by FastQC (version 0.11.5)