FastQCFastQC Report
Mon 5 Aug 2019
16C_32psu_3_S3_L001_R1_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_32psu_3_S3_L001_R1_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13683825
Sequences flagged as poor quality0
Sequence length15-145
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACTG250.008793275134.93413128-129
GCTGCTG550.00593354254.648243136-137
GGCTCTC400.006369980553.67665118-119
CAAGCTG557.6535434E-752.3363273
CTGAGCT550.007411107451.660545134-135
GCGGCTT500.00777961951.03034128-129
CAGAGCC350.0085797349.782093106-107
GATCTGC1700.047.974968
GACACTG24800.046.620832
CGATCTG6300.043.405927
CGAGTCA858.831886E-442.969543132-133
ACACTGC27300.042.3515243
CACTGCC27650.041.8154344
AGACACT27800.041.4172331
CTGCTGG1350.001075408341.283756138-139
TCTTCCG100400.040.568472
CTGCCAC29450.039.0967456
ACTGCCA29750.038.863755
TGCCACA29600.038.5744637
TTCCGAT96150.037.9209254
Produced by FastQC (version 0.11.5)