FastQCFastQC Report
Mon 5 Aug 2019
16C_32psu_2_S2_L002_R2_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_32psu_2_S2_L002_R2_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17662256
Sequences flagged as poor quality0
Sequence length15-145
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG208210.11788414798200185No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGACC406.69788E-494.71852138-139
GTAAGCC450.00808114550.541946128-129
GCCAGAG800.001009731941.816402134-135
GACACTG15100.036.047452
AGACACT15700.035.624461
TTAGCCG1101.8750658E-434.46042128-129
CACCAGC1950.00329498732.864925132-133
CACTGCC17000.032.0184334
TCTTCCG116450.031.9478572
ACACTGC17650.031.4051423
CCAGCGC650.004203762431.27037102-103
TTCCGAT115600.030.2386724
CTGCCAC18300.029.744076
ACTGCCA18400.029.5822495
GGTCCCC1100.00571041429.371704132-133
TGCCACA18750.029.0302947
CTTCCGA129400.028.2873483
GCCGATC5900.027.9310266
GGTATGC12000.027.8816532
GATCTGC2507.2759576E-1225.9674638
Produced by FastQC (version 0.11.5)