FastQCFastQC Report
Mon 5 Aug 2019
16C_32psu_2_S2_L002_R1_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_32psu_2_S2_L002_R1_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17678773
Sequences flagged as poor quality0
Sequence length15-145
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGCAT150.004606674167.5038138-139
GGCTCCG150.004606674167.5038138-139
TGACTCG550.001253497180.89431126-127
AGACACT23400.057.6106261
CTGGACT450.00512348356.713768134-135
TGGGCTC500.00629175253.84463136-137
CAGGATC700.006293582353.840508138-139
GGTCTGC653.3362524E-452.351173134-135
GACACTG25800.051.8658752
CCAGATG750.00826743350.251144138-139
ACACTGC30000.045.101983
CACTGCC30150.044.5476074
TGCCACA32250.041.6468357
CTGCCAC32150.041.6216436
ACTGCCA32350.041.2105525
CAGATGG2203.075911E-739.9725138-139
TCTTCCG125950.037.7579962
ATTGCCG750.001746703237.40504122-123
CGATCTG7000.036.95337
GCCACAT36700.036.4614688
Produced by FastQC (version 0.11.5)