FastQCFastQC Report
Mon 5 Aug 2019
16C_32psu_2_S2_L001_R2_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_32psu_2_S2_L001_R2_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17712956
Sequences flagged as poor quality0
Sequence length15-145
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG226930.12811526207144647No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180410.1018520003098297No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGCA100.008047428138.9964976-77
GAGCACT600.001286518980.366165132-133
AGGCCGA854.8919075E-543.29215126-127
GGGCCAA606.166684E-541.6257442
AGACACT14350.040.7274321
GACACTG14200.040.4531862
ACAGCGC650.001262009739.96279118-119
GCCGATC5050.037.5869186
GGGGCCA550.00201594736.3287431
CACTGCC16000.035.590014
ACACTGC16200.035.4589653
CTGCCAC16900.034.285926
CGATCTG7350.033.9802977
TGCCACA17150.033.786127
ACTGCCA17050.033.6913155
TCTTCCG119700.032.8832932
GATCTGC2650.032.044158
TTCCGAT118600.031.0824284
ATCTGCC2250.031.0807329
GTCTCAG1253.9135845E-430.39916128-129
Produced by FastQC (version 0.11.5)