FastQCFastQC Report
Mon 5 Aug 2019
16C_32psu_2_S2_L001_R1_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_32psu_2_S2_L001_R1_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17731070
Sequences flagged as poor quality0
Sequence length15-145
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACACT21950.057.828341
CAAGCTG451.1495011E-555.3085823
CAGCATG500.00876818549.509117132-133
GACACTG26950.047.2842252
TCTTCCG123250.042.205052
GGATGCC1200.001034372341.61181138-139
ACACTGC30900.041.2398033
CACTGCC31350.040.8066254
GGCACCG900.001263463939.953323136-137
TTCCGAT120400.039.689884
TGCCACA32300.039.452327
CTGCCAC32700.039.1219446
ACTGCCA32850.038.7917755
GCCGATC5200.038.290566
CTTCCGA130050.038.0461853
CGATCTG7150.036.898187
CTTCCGG4550.036.1025283
GCCACAT37050.033.856928
CCGATCT129250.033.8141946
GATCTGC2800.033.7777448
Produced by FastQC (version 0.11.5)