FastQCFastQC Report
Mon 5 Aug 2019
16C_32psu_1_S1_L002_R2_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_32psu_1_S1_L002_R2_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19541994
Sequences flagged as poor quality0
Sequence length15-145
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG269970.13814864542482205No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTCAG551.1019063E-465.5025136-137
CTGAGGC1000.006757701352.88042136-137
AGACACT25900.049.548691
CCGTGTC800.00993560547.967148138-139
GATCTGC4200.045.6565868
GACACTG28600.044.5267262
CGATCTG15850.044.050137
TCTTCCG226350.043.1405562
TTCCGAT221850.040.4473844
ATCTGCC3200.039.9495129
CACTGCC31700.039.862164
TGCCACA32150.039.610197
ACACTGC32100.039.365443
CTTCCGA238100.039.256333
CTGCCAC32800.038.675236
ACTGCCA33400.037.686045
CCGATCT219550.035.8769956
CAAGCTG1009.930009E-734.4179533
TCCGATC233550.034.2316365
GGTATGC19150.033.6348532
Produced by FastQC (version 0.11.5)