FastQCFastQC Report
Mon 5 Aug 2019
16C_32psu_1_S1_L002_R1_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_32psu_1_S1_L002_R1_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19563449
Sequences flagged as poor quality0
Sequence length15-145
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCAGC300.001389140178.82805128-129
TCGGGCA350.001979994472.09293132-133
AGACACT43800.071.1973651
AGACCGG1056.333501E-661.16014138-139
CGGGTCT700.00576652555.04413138-139
GACACTG58600.053.2159162
CCGGCAG450.00692644652.55203128-129
GTCAGCT453.839382E-450.882465108-109
CGGCTGC550.008390350.0641136-137
GGATCCA300.00916683348.9552962
TCTTCCG237300.048.3776552
ACACTGC65350.047.7186363
CACTGCC66450.047.0760544
ACTGCCA69950.044.7205665
TGCCACA69750.044.7084247
CTGCCAC69850.044.6444176
TTCCGAT229900.044.3338974
CTTCCGA246100.043.80263
GCCACAT74900.041.9611478
CTGGCAT306.940431E-540.79973210-11
Produced by FastQC (version 0.11.5)