FastQCFastQC Report
Mon 5 Aug 2019
16C_32psu_1_S1_L001_R2_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_32psu_1_S1_L001_R2_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19593518
Sequences flagged as poor quality0
Sequence length15-145
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG294010.1500547272827677No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACTGG250.003632387861.86069102-103
GGCTCAA400.004712328357.92518128-129
AGACACT23400.054.89171
GACACTG27250.047.315752
TCTTCCG222050.044.1921732
CACTGCC30200.043.345444
ACACTGC30300.042.552733
CGATCTG13750.041.875157
TGCCACA31550.041.647137
TTCCGAT218300.041.2765464
CTGCCAC32300.040.832456
ACTGCCA32350.040.465095
CTTCCGA234650.040.036323
CCGATCT216500.036.7557536
GCCACAT36200.036.2974328
GGGACCC800.002032835536.266983124-125
TCCGATC229600.035.2373435
GGTATGC19350.031.2820422
GATCTGC4450.030.965018
GGCCGAT900.00665993128.461348116-117
Produced by FastQC (version 0.11.5)