FastQCFastQC Report
Mon 5 Aug 2019
16C_32psu_1_S1_L001_R1_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_32psu_1_S1_L001_R1_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19616873
Sequences flagged as poor quality0
Sequence length15-145
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCAGC150.0017929261229.60571134-135
TGGCCTT200.0056835087156.1422128-129
AGACACT46350.070.2146761
CTGCGGC501.5675521E-460.998222126-127
CAAGCTG1400.059.515553
AGCCTGT300.004354384359.092712110-111
GACACTG61250.053.1338772
CCGGCAG450.00895121949.251124-125
TGAGCCG300.00912441249.0128025
TCTTCCG239400.048.7057042
ACACTGC67800.048.000743
CACTGCC69100.047.168624
AAGCTGG1800.046.289874
CTGCCAC72100.045.2059866
TGCCACA72000.045.2006957
ACTGCCA72050.045.169335
TTCCGAT230050.044.4428184
CTTCCGA249400.043.588773
GCCACAT79050.041.4795278
CAGCATG800.001162688440.63448130-131
Produced by FastQC (version 0.11.5)