FastQCFastQC Report
Mon 5 Aug 2019
16C_26psu_4_S16_L002_R2_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_26psu_4_S16_L002_R2_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17181036
Sequences flagged as poor quality0
Sequence length15-145
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG277060.16125919298463726No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACACT29000.054.982591
GACACTG34150.046.232762
GTTGTCC607.1462215E-444.8582130-131
TCTTCCG210200.043.6540532
ACACTGC37750.041.685783
CACTGCC38100.041.029314
TTCCGAT212650.041.0192764
CTTCCGA225500.039.790973
TGCCACA39800.039.2768027
ACTGCCA40000.039.2106865
CTGCCAC40550.038.6788526
CGATCTG14200.038.529997
CCGATCT217800.036.436786
GGCGCTA750.002140809835.886562130-131
GCCACAT43950.035.686638
GCCGATC6050.035.3123326
TCCGATC229950.035.123375
GGGCCAA600.00251117934.7381482
GTGAGCC800.003347024332.760315128-129
GCTATGC350.00403616731.53147946-47
Produced by FastQC (version 0.11.5)