FastQCFastQC Report
Mon 5 Aug 2019
16C_26psu_4_S16_L002_R1_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_26psu_4_S16_L002_R1_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17202732
Sequences flagged as poor quality0
Sequence length15-145
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACACT58450.076.04961
TGCCGCA404.4191493E-664.986267
GACACTG74400.059.676252
CGGCTCC400.005089573656.809055136-137
ACACTGC82400.054.0083
GGCTCGA350.0068782452.644978122-123
CACTGCC84450.052.450724
GCCGGTC400.007663367351.225266130-131
CTGCCAC87600.050.564656
TGCCACA87100.050.5564737
ACTGCCA87900.050.332935
TCTTCCG240550.049.6013952
GGATCCA951.5825208E-1049.253322
GCGAGCA450.00984994548.072598134-135
GCCACAT93100.047.186728
CGATCTG14700.046.3303387
TTCCGAT237250.045.886194
CAAGCTG1152.0008883E-1145.2078323
CTTCCGA252700.045.03523
CGGATCC1054.2382453E-1044.5623931
Produced by FastQC (version 0.11.5)