FastQCFastQC Report
Mon 5 Aug 2019
16C_26psu_4_S16_L001_R2_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_26psu_4_S16_L001_R2_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17245876
Sequences flagged as poor quality0
Sequence length15-145
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG301580.17487079229840222No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACACT30400.056.8005371
CAGTCCG250.00864461749.68742892-93
GGTCACC350.00984457848.0793116-117
GACACTG35600.048.0633772
CGATCTG12550.045.3072977
TCTTCCG214800.044.977692
ACACTGC39550.043.130843
CACTGCC39800.042.859924
TGCCACA40000.042.6454967
TTCCGAT215000.041.8782964
GCCGATC5650.041.5480586
CTTCCGA225750.041.362993
CTGCCAC41000.041.3510176
ACTGCCA41450.041.1537935
CCGATCT213900.038.2891356
GCCACAT44700.038.1616258
TCCGATC231200.035.8302085
GATCTGC3450.034.7772648
GGTATGC18050.032.6581572
ATCTGGC853.7042197E-430.685829
Produced by FastQC (version 0.11.5)