FastQCFastQC Report
Mon 5 Aug 2019
16C_26psu_4_S16_L001_R1_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_26psu_4_S16_L001_R1_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17268920
Sequences flagged as poor quality0
Sequence length15-145
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACACT55950.075.022281
GTCCAGT300.002220523370.0389132-133
CAGCATG350.003020264264.81136-137
TCAGCTG400.00512529856.708744136-137
CAGGAGC400.00512529856.708744136-137
GACACTG73850.056.6268422
CAAGCTG703.8797225E-852.0135843
ACACTGC80350.051.9812163
CACTGCC81700.051.1222884
GGCTGCT553.8189642E-450.937386132-133
TCTTCCG246700.049.293782
ACTGCCA84450.049.2727855
CTGCCAC84900.049.134156
TGCCACA84200.048.9866687
CGGATCC756.6804205E-848.5460131
CGATCTG14500.047.709017
TGCCACG552.90549E-547.2850767
GCCACAT88750.046.4166348
TTCCGAT241100.045.735714
CTTCCGA257800.044.6090133
Produced by FastQC (version 0.11.5)