FastQCFastQC Report
Mon 5 Aug 2019
16C_26psu_3_S15_L002_R2_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_26psu_3_S15_L002_R2_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14568496
Sequences flagged as poor quality0
Sequence length15-145
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG352700.24209774296536854No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACACT24750.057.636081
GACACTG28300.050.7935072
GGGCCAA454.733153E-448.7689971
TCTTCCG175700.046.4336242
CACTGCC31250.045.296024
ACACTGC32350.044.6037983
TGCCACA32100.044.2679637
CGATCTG11550.044.1769567
CTGCCAC32300.043.8246
ACTGCCA32300.043.8235475
TTCCGAT175950.043.685694
GCCGATC5050.043.457376
CTTCCGA184900.042.55033
CTTCCGG3500.042.3239363
GCCACAT35550.040.2805828
TTCCGGT2650.039.336714
CCGATCT185100.039.3332026
TCCGATC188100.038.7929845
CAGCTTG600.001949754236.5766226
GCCCAGA750.002518136534.71848136-137
Produced by FastQC (version 0.11.5)