FastQCFastQC Report
Mon 5 Aug 2019
16C_26psu_3_S15_L002_R1_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_26psu_3_S15_L002_R1_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14587555
Sequences flagged as poor quality0
Sequence length15-145
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACACT44050.078.724931
GACACTG55800.062.3435942
GCAGGTC450.003706561661.545532138-139
GGACCGT300.004286261359.328453130-131
ACACTGC61200.056.753123
CGCACGG300.0055706355.527306124-125
TCTTCCG203650.055.3557242
CACTGCC62450.055.354394
TGCCACA65300.053.0222247
CTGCCAC65250.052.979036
ACTGCCA65450.052.9007075
TTCCGAT202200.051.9382744
CAAGCTG852.075467E-951.483
CTTCCGA213100.051.2837143
GCCACAT68450.050.5022858
CGATCTG13000.047.1239977
GCGCCGG806.2072E-446.159153138-139
CCGATCT206050.045.977226
TCCGATC209100.045.8035935
ATCTGGC603.6089026E-545.5812459
Produced by FastQC (version 0.11.5)