FastQCFastQC Report
Mon 5 Aug 2019
16C_26psu_3_S15_L001_R2_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_26psu_3_S15_L001_R2_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14649750
Sequences flagged as poor quality0
Sequence length15-145
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG377990.2580180549156129No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCAGC100.0033377695186.55258138-139
GGTCCGC300.004113242559.949005132-133
AGACACT25350.057.192111
GGGCCAA402.6341248E-454.9174161
GACACTG27250.052.5989042
TCTTCCG178000.046.740712
ACACTGC30850.046.282933
CACTGCC30800.046.001474
TGCCACA31700.044.8688247
CTGCCAC32250.043.7630466
ACTGCCA32450.043.4931765
TTCCGAT182700.043.464284
CTTCCGA189350.042.923893
CGATCTG11800.042.8163837
GATCTGC3300.041.6036768
GCCACAT35300.040.6042488
CCGATCT191850.038.9010966
TCCGATC193150.038.667575
CGTAGCA2051.439761E-436.04383138-139
GCCGATC5400.035.5941356
Produced by FastQC (version 0.11.5)