FastQCFastQC Report
Mon 5 Aug 2019
16C_26psu_3_S15_L001_R1_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_26psu_3_S15_L001_R1_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14670575
Sequences flagged as poor quality0
Sequence length15-145
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCATT153.771013E-4109.4401868
GGGCCAT153.771013E-4109.4401867
TGGGCCA200.001183155682.080146
AGACACT46050.077.59441
GGACCGC250.002079890871.203926130-131
CTGGGCC250.002867554365.664115
AGATGCC300.002954589265.17053136-137
GACACTG57550.062.0892
ACACTGC64750.055.18493
CACTGCC65200.054.8879434
TCTTCCG203300.054.2086872
TGCCACA68400.052.400097
CTGCCAC68800.052.0954366
GGTCTGC300.007263052751.925194118-119
ACTGCCA69000.051.865135
CGATCTG12850.051.5263147
TTCCGAT200150.051.152284
CTTCCGA212550.049.815753
GCCACAT72450.049.3198368
CAAGCTG454.7953427E-448.6400833
Produced by FastQC (version 0.11.5)