FastQCFastQC Report
Mon 5 Aug 2019
16C_26psu_2_S14_L002_R2_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_26psu_2_S14_L002_R2_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17151888
Sequences flagged as poor quality0
Sequence length15-145
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG173140.1009451554254552No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCCAG100.0089118285134.3321134-135
GGACCCG402.7124368E-454.59269118-119
AGACACT25000.053.4815181
TTGCCAC650.007588301351.35281120-121
GACACTG27750.048.1814042
ACACTGC31100.043.1648033
CACTGCC30900.042.920764
TGCCACA31250.042.095017
CTGCCAC31950.041.341486
TCTTCCG207900.041.2319072
ACTGCCA32850.040.2088365
TTCCGAT205350.039.7748644
CGATCTG13100.038.685427
CTTCCGA218150.038.4296073
GATCTGC3800.038.3063668
GCCACAT36250.036.1400768
CCGATCT213450.035.310336
TCCGATC224900.033.824265
TGGGACC650.003328616732.797268116-117
GCCGATC5450.032.6443676
Produced by FastQC (version 0.11.5)