FastQCFastQC Report
Mon 5 Aug 2019
16C_26psu_2_S14_L002_R1_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_26psu_2_S14_L002_R1_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17166593
Sequences flagged as poor quality0
Sequence length15-145
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCAGC106.8379735E-4316.80212138-139
AGCTGCG350.00103008784.99385138-139
AGACACT40600.069.120411
TGGAGCC250.003455777262.643913114-115
TCAGGCT350.005267166556.31891132-133
GTCAGGC350.005832578554.886158130-131
GACACTG51200.054.389512
ACACTGC56200.049.741843
GACTGTC804.363395E-449.579742138-139
CACTGCC56550.049.3387264
TCTTCCG209550.047.2446252
CTGCCAC59500.046.801996
TGCCACA59600.046.5427177
ACTGCCA60000.046.2324335
TTCCGAT205400.044.3176844
CGATCTG11300.043.853097
GCCACAT64550.043.224068
CTTCCGA221000.042.9198153
CCGATCT201200.039.808316
CAAGCTG709.808253E-538.473593
Produced by FastQC (version 0.11.5)