FastQCFastQC Report
Mon 5 Aug 2019
16C_26psu_2_S14_L001_R2_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_26psu_2_S14_L001_R2_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17220103
Sequences flagged as poor quality0
Sequence length15-145
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG183880.10678217197655554No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACCC300.00493429857.255604122-123
GGGTCCT150.005584505455.49257342-43
AGACACT25800.052.739241
AGGGCAC300.007269682451.913246114-115
GACACTG30150.044.9500922
CGATCTG10850.042.7972077
TCTTCCG200050.042.131662
ACACTGC33100.041.106973
CACTGCC33500.040.6161424
TTCCGAT200150.039.6556134
TGCCACA35150.038.556287
CTTCCGA214450.038.043583
ACTGCCA35950.037.8483625
CTGCCAC35850.037.8033266
TCCGGTC751.4511804E-435.9965
CAAGCTG751.4512299E-435.995793
CCGATCT204800.035.7235686
GATCTGC3250.034.8888328
GGTATGC15350.034.1198842
TCCGATC222100.033.3542375
Produced by FastQC (version 0.11.5)