FastQCFastQC Report
Mon 5 Aug 2019
16C_26psu_2_S14_L001_R1_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_26psu_2_S14_L001_R1_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17236176
Sequences flagged as poor quality0
Sequence length15-145
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGAC650.001932045172.539406138-139
AGACACT42400.068.42061
CCAGGAA700.006058275254.361683130-131
GACACTG53600.054.324962
CAAGCTG606.22842E-753.9225543
ACACTGC59450.048.8885733
CACTGCC60500.047.6835754
TCTTCCG219350.047.3957982
CTGCCAC63750.045.1680686
TGCCACA63700.045.1188747
ACTGCCA64200.045.019455
TTCCGAT212550.044.9544874
CGATCTG12600.044.935467
CTTCCGA225650.044.0891273
GCCACAT67950.041.979448
GATCTGC3600.041.9397628
CCGATCT206650.041.1497046
GCCGATC5650.040.084026
TCCGATC229100.037.588095
CTGCATG951.8849364E-434.429703132-133
Produced by FastQC (version 0.11.5)