FastQCFastQC Report
Mon 5 Aug 2019
16C_26psu_1_S13_L002_R2_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_26psu_1_S13_L002_R2_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22028227
Sequences flagged as poor quality0
Sequence length15-145
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG257280.1167956004811463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACACT29350.050.4544831
GATCTGC4350.046.531558
GACACTG32350.045.4449922
TCTTCCG257200.041.5856972
CGATCTG16200.040.77187
ACACTGC36700.040.4938773
CACTGCC36250.040.2618564
TTCCGAT257500.038.951514
TGCCACA37950.038.037397
CTGCCAC38050.037.9372526
CTTCCGA274850.037.4229283
ACTGCCA39200.036.960185
CCGATCT264450.034.5237926
CGGCACA700.002954528433.605057122-123
ATCTGCC3500.033.4817249
TCCGATC286850.032.2735065
GCCACAT44750.031.9003168
GCAGCAC600.00422863831.232746104-105
GGGCCCA750.006719728.409381
CTTCCGG7900.027.6444533
Produced by FastQC (version 0.11.5)