FastQCFastQC Report
Mon 5 Aug 2019
16C_26psu_1_S13_L002_R1_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_26psu_1_S13_L002_R1_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22051389
Sequences flagged as poor quality0
Sequence length15-145
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACACT51650.070.5256961
GACACTG63900.056.5900232
GGCCGGC400.005722829655.15034136-137
ACACTGC69950.051.7712063
TGGACAC400.00774194951.093304132-133
CACTGCC71150.050.8980454
CAAGCTG951.2914825E-1050.3049283
TCTTCCG274650.049.8808332
CGATCTG15050.049.7478457
CTGCCAC73650.048.9540566
ACTGCCA74000.048.7942735
TGCCACA74000.048.7225157
TTCCGAT270100.046.2189064
CTTCCGA287900.045.1688233
GATCTGC4250.044.9785238
GCCACAT80250.044.795598
CCGATCT266100.041.785296
TCCGATC291650.038.6526835
AGACCGG1100.001789080437.22287138-139
CTTCCGG8500.035.6079983
Produced by FastQC (version 0.11.5)