FastQCFastQC Report
Mon 5 Aug 2019
16C_26psu_1_S13_L001_R2_001_trimmed.5bp_3prime.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16C_26psu_1_S13_L001_R2_001_trimmed.5bp_3prime.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22129995
Sequences flagged as poor quality0
Sequence length15-145
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG276300.12485316874224328No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCAGC350.003503994662.42517136-137
GGGCCAC300.006531717753.3377042
AGACACT29650.049.830511
GACACTG32850.044.488682
TCTTCCG256200.041.7832832
ACACTGC37550.039.6302343
TTCCGAT259200.039.3446124
CACTGCC38000.039.1609274
CGATCTG14650.037.8645677
TGCCACA39700.037.3500827
ACTGCCA40000.037.203225
CTGCCAC39800.037.1222236
CTTCCGA281650.037.0606843
CCGATCT266100.034.8772056
GCCGATC5950.034.0646486
GATCTGC4950.032.3261768
TCCGATC293450.032.0445755
GCCACAT46650.031.9000118
TGCTCGC700.003820561331.887035118-119
GGTATGC19850.028.4826052
Produced by FastQC (version 0.11.5)